Hydroxamate Siderophores Secreted by Plant Endophytic Pseudomonas putida Elicit Defense against Blast Disease in Rice Incited by Magnaporthe oryzae.
Mushineni AshajyothiVelmurugan ShanmugamAditi KunduAlexander BalamuruganVinod ChouhanKuleshwar Prasad SahuPublished in: Letters in applied microbiology (2023)
Our study focuses on a hydroxamate-type siderophore from Pseudomonas putida BP25, known for chelating ferric iron and aiding microbial growth in iron-deficient environments. Confirmed through CAS-agar and tetrazolium tests, a purified siderophore extract was obtained via ion-exchange chromatography. Applying varying concentrations of this siderophore to rice seedlings demonstrated concentration-dependent effects on shoot and root phenotypes. Prophylactic application on rice leaves significantly reduced blast severity (68.7-97.0%), surpassing curative application (47.5-86.87%). Additionally, the siderophore treatment elevated peroxidase, polyphenol oxidase, and total phenols in rice plants. Defense-related genes linked to salicylic acid (OsPR1.1, OsNPR1, OsPDF2.2), and other pathways (Oshox24, OsCLE, OsGLP3-3, OsEIN2.4, OsCSE) promoting blast suppression showed upregulation. However, the OsACS6 gene associated with ethylene-induced internodal elongation was significantly downregulated. Overall, our findings propose that the siderophore from Pseudomonas putida BP25 induces defense gene transcription, offering potential for sustainable rice production via bio-formulation.
Keyphrases
- copy number
- biofilm formation
- crispr cas
- genome wide
- mass spectrometry
- microbial community
- oxidative stress
- cell proliferation
- iron deficiency
- gene expression
- staphylococcus aureus
- rectal cancer
- escherichia coli
- risk assessment
- cystic fibrosis
- pseudomonas aeruginosa
- dna methylation
- high glucose
- liquid chromatography
- genome wide identification
- combination therapy
- arabidopsis thaliana
- prognostic factors
- tandem mass spectrometry
- replacement therapy
- type iii