In silico gepotidacin target mining among 33 213 global Neisseria gonorrhoeae genomes from 1928 to 2023 combined with gepotidacin MIC testing of 22 gonococcal isolates with different GyrA and ParC substitutions.

Alexandra DavidDaniel GolparianSusanne JacobssonCaleb StrattonPham Thi LanKen ShimutaPam SonnenbergNigel FieldMakoto OhnishiChristopher DaviesMagnus Unemo

Published in: The Journal of antimicrobial chemotherapy (2024)

In silico mining of 33 213 global gonococcal genomes (isolates from 1928 to 2023) showed that A92 is highly conserved in GyrA, while alterations in D86 of ParC are common. No GyrA plus ParC alterations caused gepotidacin resistance. MIC determination and genomic surveillance of potential antimicrobial resistance determinants are imperative.

Keyphrases

antimicrobial resistance
molecular docking
public health
transcription factor
genetic diversity
gene expression
risk assessment
molecular dynamics simulations