NINscope, a versatile miniscope for multi-region circuit investigations.
Andres de GrootBastijn J G van den BoomRomano M van GenderenJoris CoppensJohn van VeldhuijzenJoop BosHugo HoedemakerMario NegrelloIngo WilluhnChris I De ZeeuwTycho M HooglandPublished in: eLife (2020)
Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.
Keyphrases
- functional connectivity
- resting state
- subarachnoid hemorrhage
- body mass index
- weight loss
- single molecule
- physical activity
- weight gain
- spinal cord
- deep learning
- cerebral ischemia
- body weight
- neuropathic pain
- living cells
- parkinson disease
- high fat diet induced
- high throughput
- white matter
- machine learning
- multiple sclerosis
- metabolic syndrome
- spinal cord injury
- blood brain barrier
- radiation therapy
- energy transfer