Endophytic interaction of Bacillus sp. in micropropagated banana plantlets.

The banana tree is associated with different species of endophytic bacteria that can stimulate plant growth. However, further studies are needed to better understand the relationships between this group of bacteria and the host plant. The objective of this study was to investigate the localization of the EB-40 (Bacillus sp.) through anatomical and ultrastructural analyses in micropropagated banana plantlets. The results demonstrated the effective colonization of the EB-40 isolate in the intercellular and intracellular spaces, as well as in the rhizosphere region. The wall of endophytic bacteria contains calcium and nitrogen. The EB-40 isolate was also observed to associate with the plasma membrane and cell wall. These results further our understanding of the mechanisms involved in the colonization of plant cells by endophytic bacteria in micropropagated banana plantlets.