Nanozyme-Based Biofuel Cell Ingeniously Coupled with Luminol Chemiluminescence System through In Situ Co-Reactant Generation for Dual-Signal Biosensing.
Cui WangYuqing WangJunhua LiuFeng LiPanpan GaiPublished in: Analytical chemistry (2023)
Classical luminol-based chemiluminescence (CL) is the process of emitting light enhanced by the addition of coreactant hydrogen peroxide (H 2 O 2 ). To address the instability issue of H 2 O 2 decomposition, herein, we proposed a nanozyme-based biofuel cell (BFC) ingeniously coupled with a luminol CL system via in situ generation of H 2 O 2 . Specifically, the gold nanoparticle (AuNP) nanozyme with glucose oxidase-like activity can act as the anodic enzyme of BFC to catalyze the oxidation of glucose to produce H 2 O 2 and electrons. In this case, H 2 O 2 as a coreactant enhanced the CL intensity and the cathode of the BFC obtained electrons to generate the open circuit voltage ( E OCV ) signals. As a result, a dual-signal biosensing platform was successfully constructed. Interestingly, the AuNPs-catalyzed system operates in an alkaline medium, which precisely meets the pH requirement for luminol luminescence. Such a BFC-CL system not only greatly lessens the effect of unstable exogenous H 2 O 2 on the signal stability but also enhances the CL of luminol. Furthermore, both CL and E OCV signals present a positive correlation with the glucose concentration. Therefore, this novel BFC-CL system shows good performance for dual-signal biosensing, which would serve as a valuable guideline for the design and application of BFC-based self-powered or CL biosensors.