Resistance gene cloning from a wild crop relative by sequence capture and association genetics.
Sanu AroraBurkhard SteuernagelKumar GauravSutha ChandramohanYunming LongOadi MatnyRyan JohnsonJacob EnkSambasivam PeriyannanNarinder SinghMuhammad Asyraf Md HattaNaveenkumar AthiyannanJitender CheemaGuotai YuNgonidzashe KangaraSreya GhoshLes J SzaboJesse A PolandHarbans BarianaJonathan D G JonesAlison R BentleyMick AyliffeEric OlsonSteven S XuBrian J SteffensonEvans S LagudahBrande B H WulffPublished in: Nature biotechnology (2019)
Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.