Resistance gene cloning from a wild crop relative by sequence capture and association genetics.

Sanu AroraBurkhard SteuernagelKumar GauravSutha ChandramohanYunming LongOadi MatnyRyan JohnsonJacob EnkSambasivam PeriyannanNarinder Singh Muhammad Asyraf Md Hatta Naveenkumar AthiyannanJitender CheemaGuotai YuNgonidzashe KangaraSreya GhoshLes J SzaboJesse A PolandHarbans BarianaJonathan D G JonesAlison R BentleyMick AyliffeEric OlsonSteven S XuBrian J Steffenson Evans S Lagudah Brande B H Wulff

Published in: Nature biotechnology (2019)

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

Keyphrases

genome wide
genome wide identification
copy number
climate change
dna methylation
genome wide analysis
transcription factor
gene expression
single cell
bioinformatics analysis
amino acid