Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM).
Yue ZhuoJi Sun ChoiThibault MarinHojeong YuBrendan A C HarleyBrian T CunninghamPublished in: Light, science & applications (2018)
Focal adhesions are critical cell membrane components that regulate adhesion and migration and have cluster dimensions that correlate closely with adhesion engagement and migration speed. We utilized a label-free approach for dynamic, long-term, quantitative imaging of cell-surface interactions called photonic resonator outcoupler microscopy (PROM) in which membrane-associated protein aggregates outcoupled photons from the resonant evanescent field of a photonic crystal biosensor, resulting in a highly localized reduction of the reflected light intensity. By mapping the changes in the resonant reflected peak intensity from the biosensor surface, we demonstrate the ability of PROM to detect focal adhesion dimensions. Similar spatial distributions can be observed between PROM images and fluorescence-labeled images of focal adhesion areas in dental epithelial stem cells. In particular, we demonstrate that cell-surface contacts and focal adhesion formation can be imaged by two orthogonal label-free modalities in PROM simultaneously, providing a general-purpose tool for kinetic, high axial-resolution monitoring of cell interactions with basement membranes.
Keyphrases
- label free
- cell surface
- biofilm formation
- high resolution
- stem cells
- high speed
- single molecule
- cell migration
- deep learning
- optical coherence tomography
- cell adhesion
- convolutional neural network
- social media
- cell therapy
- staphylococcus aureus
- machine learning
- energy transfer
- high throughput
- gold nanoparticles
- high intensity
- mass spectrometry
- escherichia coli
- bone marrow
- candida albicans
- pet ct
- sensitive detection
- oral health
- quantum dots
- high density