A Multiplex Quantitative Reverse Transcription Polymerase Chain Reaction Assay for the Detection of KIAA1549-BRAF Fusion Transcripts in Formalin-Fixed Paraffin-Embedded Pilocytic Astrocytomas.
David BretValentin ChappuisDelphine PoncetFrançois DucrayKaren SilvaFabrice MionAlexandre VasiljevicCarole Ferraro-PeyretCarmine MottolesePierre LeblondMathieu GabutDidier FrappazNathalie StreichenbergerDavid MeyronetPierre-Paul BringuierMarc BarritaultPublished in: Molecular diagnosis & therapy (2020)
The results show that it could be beneficial to implement the multiplex method in a clinical setting, where samples presenting low quantity of degraded RNA are not unusual.