Modulation of Angiogenic Activity by Light-Activatable miRNA-Loaded Nanocarriers.
Miguel M LinoSusana SimõesAndreia VilaçaHelena AntunesAlessandra ZonariLino S FerreiraPublished in: ACS nano (2018)
The combinatorial delivery of miRNAs holds great promise to modulate cell activity in the context of angiogenesis. Yet, the delivery of multiple miRNAs with spatiotemporal control remains elusive. Here, we report a plasmonic nanocarrier to control the release of two microRNAs. The nanocarrier consists of gold nanorods modified with single-stranded DNA for hybridization with complementary DNA-conjugated microRNAs. DNA strands with distinct melting temperatures enable the independent release of each microRNA with a near-infrared laser using the same wavelength but different powers. Tests in human outgrowth endothelial cells (OECs) indicate that this system can be used to silence different targets sequentially and, by doing so, to modulate cell activity with spatiotemporal resolution. Finally, using an in vivo acute wound healing animal model, it is demonstrated that the order by which each miRNA was released in transplanted OECs significantly impacted the wound healing kinetics.
Keyphrases
- wound healing
- endothelial cells
- single molecule
- drug delivery
- circulating tumor
- cell free
- nucleic acid
- single cell
- cell therapy
- cancer therapy
- liver failure
- vascular endothelial growth factor
- stem cells
- intensive care unit
- drug release
- hepatitis b virus
- mass spectrometry
- machine learning
- energy transfer
- drug induced
- respiratory failure
- deep learning