Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization.
Jason A WestMari MitoSatoshi KurosakaToru TakumiChiharu TanegashimaTakeshi ChujoKaori YanakaRobert E KingstonTetsuro HiroseCharles S BondArcha H FoxShinichi NakagawaPublished in: The Journal of cell biology (2016)
Paraspeckles are nuclear bodies built on the long noncoding RNA Neat1, which regulates a variety of physiological processes including cancer progression and corpus luteum formation. To obtain further insight into the molecular basis of the function of paraspeckles, we performed fine structural analyses of these nuclear bodies using structural illumination microscopy. Notably, paraspeckle proteins are found within different layers along the radially arranged bundles of Neat1 transcripts, forming a characteristic core-shell spheroidal structure. In cells lacking the RNA binding protein Fus, paraspeckle spheroids are disassembled into smaller particles containing Neat1, which are diffusely distributed in the nucleoplasm. Sequencing analysis of RNAs purified from paraspeckles revealed that AG-rich transcripts associate with Neat1, which are distributed along the shell of the paraspeckle spheroids. We propose that paraspeckles sequester core components inside the spheroids, whereas the outer surface associates with other components in the nucleoplasm to fulfill their function.
Keyphrases
- long noncoding rna
- binding protein
- high resolution
- single molecule
- single cell
- induced apoptosis
- high throughput
- high speed
- papillary thyroid
- cell cycle arrest
- air pollution
- squamous cell carcinoma
- label free
- young adults
- neural network
- mass spectrometry
- cell proliferation
- highly efficient
- childhood cancer
- solar cells