LC-MS Approach to Decipher a Light Chain Chromatographic Peak Splitting of a Monoclonal Antibody.
Yan-Jun LiuZachary L VanAernumYue ZhangXinliu GaoMariana VladBo FengRobert CrossBruce KilgoreAlice NewmanDongdong WangHillary A SchuesslerDouglas D RichardsonJennifer S ChadwickPublished in: Pharmaceutical research (2023)
Liquid chromatography-mass spectrometry (LC-MS) has been utilized extensively to assess PQAs of biological therapeutics. In this study, a generalizable LC-MS-based approach was developed to enable identification and quantitation of the isoAsp-containing peptides.
Keyphrases
- liquid chromatography
- mass spectrometry
- monoclonal antibody
- simultaneous determination
- tandem mass spectrometry
- high resolution mass spectrometry
- high performance liquid chromatography
- gas chromatography
- high resolution
- multidrug resistant
- liquid chromatography tandem mass spectrometry
- solid phase extraction
- small molecule