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Biomechanical and histological comparison of two suture configurations for soft tissue grafts: speedtrap™ versus krackow stitch.

Anell Olivos-MezaEdgar Manuel Hernández-EspinozaVíctor Manuel Domínguez-HernándezVíctor Manuel Araujo-MonsalvoEréndira Georgina Estrada-VillaseñorRaúl Pichardo-BahenaLuis Ángel Murguía-GonzálezJuan Diego Rodríguez-ReinosoJonatan de Jesus Hernández-LeónCarlos Landa-Solís
Published in: Cell and tissue banking (2024)
To compare 2 different graft preparation techniques to determine biomechanical strength and resultant tissue trauma evaluated by histology. Twelve common flexors of the finger's tendons were prepared with either tubulization (SpeedTrap™) or transtendon stiches (Orthocord™). The stiffness, resistance and energy at maximum load were tested for biomechanical assessment in both groups. After load testing, Samples were stained with hematoxylin and eosin (HE) to evaluate histological damage. We observe that the time to prepare tendons with SpeedTrap™ was 8.3 times faster (1:25 min) than traditional ones (15:02 min). In all cases, the mean values for SpeedTrap™ were higher in terms of strength, stiffness and energy at maximum load than for traditional suture but without significant difference (p > 0.05). The Krackow stitch produces greater structural damage to the collagen fibers while SpeedTrap™ maintains better organized arrangement of the fibers after tubulization preparation. With the results obtained, we can conclude that the tubulization technique allows faster graft preparation with less structural damage to the manipulated tissue without altering the biomechanical resistance provided by the transtendon suture technique.
Keyphrases
  • finite element analysis
  • finite element
  • oxidative stress
  • molecularly imprinted
  • soft tissue
  • mass spectrometry