Sensitive detection of antibiotics using aptamer conformation cooperated enzyme-assisted SERS technology.
Qianqian FangYingying LiXinxing MiaoYiqiu ZhangJun YanTainrong YuJian LiuPublished in: The Analyst (2019)
Serious healthcare concerns have been raised on the issue of antibiotic residues after overuse, especially by accumulation in the human body through food webs. Here, we report a methodological development for sensitive detection of antibiotics with aptamer conformation cooperated enzyme-assisted SERS (ACCESS) technology. We design and integrate a set of nucleic acid oligos, realizing specific recognition of chloramphenicol (CAP) and efficient exonuclease III-assisted DNA amplification. It features a "signal-on" analysis of CAP with the limit of detection (15 fM), the lowest concentration detectable in the literature. Our method exhibits a high selectivity on the target analyte, free of interference of other potential antibiotic contaminants. The ACCESS assay promises an ultrasensitive and specific detection tool for trace amounts of antibiotic residues in samples of our daily life.
Keyphrases
- sensitive detection
- nucleic acid
- loop mediated isothermal amplification
- quantum dots
- label free
- healthcare
- endothelial cells
- gold nanoparticles
- molecular dynamics simulations
- systematic review
- human health
- physical activity
- crystal structure
- induced pluripotent stem cells
- mass spectrometry
- health information
- molecularly imprinted
- social media
- single cell
- simultaneous determination