HIF1 α : A Novel Biomarker with Potential Prognostic and Immunotherapy in Pan-cancer.

Cancer is a catastrophic disease that seriously affects human health. HIF1 α plays an important role in cancer initiation, progression, and prognosis. However, little is known about the specific role of HIF1 α in pan-cancer. Therefore, we systematically and comprehensively analyzed HIF1 α using GEPIA, HPA, GeneMANIA, STRING, SMPDB, cBioPortal, UALCAN, and TISDB databases and also 33 cancer and normal tissues in TCGA downloaded from the Genome Data Commons (GDC) data portal. Data and statistical analysis were performed using R software v4.0.3. Our results found that there were differences in the mRNA expression levels of HIF1 α in human pan-cancer and its corresponding normal tissues. The expression level of HIF1 α correlated with tumor stage in LIHC and also significantly correlated with prognosis in LIHC, LUSC, STAD, OV, PAAD, PRAD, THCA, LUAD, MESO, and READ. The small molecule pathways involved in HIF1 α include succinate signaling, fumarate, and succinate carcinogenesis-related pathways. The highest mutation frequency of the HIF1 α gene in pan-cancer was head and neck cancer, and the HIF1 α methylation level in most tumors is significantly reduced. HIF1 α was not only associated with immune cell infiltration but also with immune checkpoint genes and immune regulators TMB and MSI. There were currently 5 small molecule drugs targeting HIF1 α .