Cost-Effective Trap qPCR Approach to Evaluate Telomerase Activity: an Important Tool for Aging, Cancer, and Chronic Disease Research.
Thalyta Nery Carvalho PintoJuliana Ruiz FernandesLiã Bárbara ArrudaAlberto Jose da Silva DuarteGil BenardPublished in: Clinics (Sao Paulo, Brazil) (2021)
Telomeres and telomerase are implicated in aging and development of chronic diseases and cancer; however, difficulty in accessing commercial kits to investigate these aspects is a critical constraint in health surveillance studies. Our optimized assay was successfully used to differentiate telomerase activity between unstimulated and stimulated cells, clearly showing the reactivation of telomerase upon cell activation. This assay is affordable, reproducible, and can be executed in resource-limited settings.
Keyphrases
- papillary thyroid
- public health
- high throughput
- healthcare
- squamous cell
- induced apoptosis
- mental health
- single cell
- squamous cell carcinoma
- cell therapy
- childhood cancer
- health information
- cell death
- signaling pathway
- lymph node metastasis
- young adults
- endoplasmic reticulum stress
- bone marrow
- case control
- health promotion