[Determination of thiabendazole in the conditions of chemical and toxicological analysis of biomaterial].

Is to study the features and to develop the methods for the determination of thiabendazole in the tissues of cadaveric organs and blood. When performing the experiments the methods of TLC, GC-MS and spectrophotometry were used. The advantages were substantiated and the optimal conditions for the release of thiabendazole from the tissues of organs and blood with acetone were determined. A variant of purification of the substance extracted from biomatrixes by the method of column chromatography of normal pressure (sorbent L 40/100 μm, mobile phase acetone-dichloromethane (9.5: 0.5)) is proposed. To identify the analyte by TLC the Sorbfil plates were used and a toluene-acetonitrile mobile phase (2:8). When identifying thiabendazole by a combination of gas-liquid chromatography and mass spectrometry (fragmentation of molecules by electron impact 70 eV) an HP-5MS column 30 m × 0.25 mm with a non-polar stationary phase (5% -phenyl) -methylpolysiloxane was proposed. The expediency of the spectrophotometric determination of thiabendazole on the basis of absorption in an acetonitrile medium has been shown. Methods for the determination of thiabendazole in tissues of organs and blood have been developed and validated. It is shown that the techniques meet the requirements of linearity, selectivity, correctness, precision and stability. The limits of detection of thiabendazole in the liver and blood are 0.14 and 0.10 mg, respectively; the limits of quantitative determination are 0.26 and 0.18 mg per 100 g of biomatrix.