Spectral libraries from nucleobases and deoxyribonucleosides facilitate the identification of ribonucleosides by nano-flow liquid chromatography-tandem mass spectrometry.
Guadalupe EspadasLaia LloveraAlexane OllivierFrancesca TuortoEva Maria NovoaEduard SabidóPublished in: Rapid communications in mass spectrometry : RCM (2024)
The study demonstrates the efficacy of utilizing homologous nucleobases and deoxyribonucleosides as interchangeable reference spectral libraries for identifying modified ribonucleosides and their positional isomers. This approach offers a valuable solution for overcoming limitations posed by the unavailability of commercial standards, enhancing the analysis of RNA post-transcriptional modifications via mass spectrometry.
Keyphrases
- liquid chromatography tandem mass spectrometry
- mass spectrometry
- optical coherence tomography
- simultaneous determination
- solid phase extraction
- ms ms
- liquid chromatography
- high performance liquid chromatography
- dna repair
- dna damage
- transcription factor
- dual energy
- magnetic resonance imaging
- computed tomography
- gas chromatography
- tandem mass spectrometry
- magnetic resonance
- capillary electrophoresis
- heat stress