Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis.
Meabh CullinaneLauren ScofieldGerald L MurrayMatthew S PayneCatherine M BennettSuzanne Marie GarlandLisa Helen Amirnull nullPublished in: The Australian & New Zealand journal of obstetrics & gynaecology (2022)
Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase-negative staphylococci (CoNS) are a possible alternative pathogen. This case-control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain-level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases.
Keyphrases
- biofilm formation
- staphylococcus aureus
- polycystic ovary syndrome
- candida albicans
- circulating tumor
- pseudomonas aeruginosa
- cell free
- liver failure
- genetic diversity
- endothelial cells
- preterm infants
- type diabetes
- intensive care unit
- gene expression
- copy number
- cystic fibrosis
- single cell
- pregnant women
- pregnancy outcomes
- hepatitis b virus
- insulin resistance
- circulating tumor cells
- aortic dissection
- breast cancer risk