Plasmodium falciparum histidine-rich protein II causes vascular leakage and exacerbates experimental cerebral malaria in mice.
Priya PalAmanda E BalabanMichael S DiamondPhotini SinnisRobyn S KleinDaniel E GoldbergPublished in: PloS one (2017)
A devastating complication of Plasmodium falciparum infection is cerebral malaria, in which vascular leakage and cerebral swelling lead to coma and often death. P. falciparum produces a protein called histidine-rich protein II (HRPII) that accumulates to high levels in the bloodstream of patients and serves as a diagnostic and prognostic marker for falciparum malaria. Using a human cerebral microvascular endothelial barrier model, we previously found that HRPII activates the endothelial cell inflammasome, resulting in decreased integrity of tight junctions and increased endothelial barrier permeability. Here, we report that intravenous administration of HRPII induced blood-brain barrier leakage in uninfected mice. Furthermore, HRPII infusion in P. berghei-infected mice increased early mortality from experimental cerebral malaria. These data support the hypothesis that HRPII is a virulence factor that contributes to cerebral malaria by compromising the integrity of the blood-brain barrier.
Keyphrases
- plasmodium falciparum
- endothelial cells
- blood brain barrier
- subarachnoid hemorrhage
- cerebral ischemia
- high glucose
- brain injury
- end stage renal disease
- staphylococcus aureus
- protein protein
- chronic kidney disease
- high fat diet induced
- pseudomonas aeruginosa
- ejection fraction
- newly diagnosed
- cerebral blood flow
- low dose
- binding protein
- small molecule
- peritoneal dialysis
- metabolic syndrome
- vascular endothelial growth factor
- cardiovascular events
- prognostic factors
- antimicrobial resistance
- skeletal muscle
- patient reported