This method is the first time to determine the metabolites of KYN, 5-HT and NAD pathway at the same time, and it is found that TRY-NAD metabolic network will be disordered after sleep deprivation. This work clarifies the importance of the pH of the extraction solution, the time and temperature control in pretreatment in standardized protocols building, and overcoming the problems of inconsistent sample pretreatment, separation, matrix effect interference and potential metabolite degradation. This method exhibits great prospects in providing more information on metabolic disturbances caused by sleep deprivation as well as neurological diseases and ageing.
Keyphrases
- sleep quality
- physical activity
- mental health
- ms ms
- liquid chromatography
- metabolic syndrome
- type diabetes
- drug delivery
- cancer therapy
- simultaneous determination
- adipose tissue
- high resolution
- skeletal muscle
- depressive symptoms
- human health
- high fat diet induced
- social media
- solid phase extraction
- wild type
- high resolution mass spectrometry