Self-Powered Engineering of Cell Membrane Receptors to On-Demand Regulate Cellular Behaviors.
Hongyan GengShuangcheng ZhiXuemin ZhouYongcun YanGuofang ZhangSenquan DaiShuzhen LvSai BiPublished in: Nano letters (2024)
On-demand engineering of cell membrane receptors to nongenetically intervene in cellular behaviors is still a challenge. Herein, a membraneless enzyme biofuel cell-based self-powered biosensor (EBFC-SPB) was developed for autonomously and precisely releasing Zn 2+ to initiate DNAzyme-based reprogramming of cell membrane receptors, which further mediates signal transduction to regulate cellular behaviors. The critical component of EBFC-SPB is a hydrogel film on a biocathode which is prepared using a Fe 3+ -cross-linked alginate hydrogel film loaded with Zn 2+ ions. In the working mode in the presence of glucose/O 2 , the hydrogel is decomposed due to the reduction of Fe 3+ to Fe 2+ , accompanied by rapid release of Zn 2+ to specifically activate a Zn 2+ -responsive DNAzyme nanodevice on the cell surface, leading to the dimerization of homologous or nonhomologous receptors to promote or inhibit cell proliferation and migration. This EBFC-SPB platform provides a powerful "sensing-actuating-treating" tool for chemically regulating cellular behaviors, which holds great promise in precision biomedicine.
Keyphrases
- drug delivery
- wound healing
- heavy metals
- single cell
- cell surface
- cell therapy
- tissue engineering
- hyaluronic acid
- cancer therapy
- living cells
- room temperature
- quantum dots
- oxidative stress
- gold nanoparticles
- stem cells
- type diabetes
- dna repair
- metabolic syndrome
- risk assessment
- machine learning
- deep learning
- big data
- mesenchymal stem cells
- visible light
- bone marrow
- blood glucose
- fluorescent probe