A PCR-free screen-printed magnetic electrode for the detection of circular RNA from hepatocellular cancer based on a back-splice junction.
Bin ZhangYitao LiangLiang BoMingyu ChenBobo HuangQingpeng CaoJinwei WeiTianyu LiXiujun CaiXuesong YePublished in: RSC advances (2021)
Circular RNA (circRNA) has the potential to be applied to disease diagnosis and therapy. However, the currently available circRNA detection techniques are limited. This work proposes a sensitive and selective approach for circRNA detection based on gold nanoparticle-modified screen-printed magnetic electrodes (AuNPs-SPME). Magnetic beads (MBs) with capture probes based on specific back-splice junction (BSJ) sites were employed to identify and selectively isolate the target circRNA, which could be directly adsorbed onto the AuNPs-SPME. Then, the circRNA attached to the surface was detected by changes in the methylene blue redox signal. The simple and time-saving AuNPs-SPME is highly sensitive (LOD = 1.0 pM) to circCDYL, one of the biomarkers of hepatocellular cancer (HCC). The analytical performance of the method presented has also been verified in human serum samples, holding great promise for clinical diagnosis.
Keyphrases
- molecularly imprinted
- real time pcr
- papillary thyroid
- loop mediated isothermal amplification
- label free
- squamous cell
- high throughput
- small molecule
- air pollution
- living cells
- particulate matter
- low cost
- risk assessment
- carbon nanotubes
- fluorescence imaging
- high resolution
- gold nanoparticles
- liquid chromatography
- fluorescent probe
- solid state
- human health
- bone marrow
- sensitive detection