Fluorescent imaging using novel conjugated polymeric nanoparticles-affimer probes in complex in vitro models of colorectal cancer.

Precious JolugboThomas WillottWei-Hsiang LinThomas MaiseyDermott O'CallaghanMark A GreenDavid G JayneM Ibrahim Khot

Published in: Nanoscale (2023)

We developed a carcinoembryonic antigen (CEA) conjugated polymer nanoparticle (CPN510-CEA-Af) probe to target CEA-expressing CRC cells in vitro . Its efficacy was evaluated in 2D and 3D cultures of LS174T, LoVo, and HT29 CRC cell lines. CPN510-CEA-Af produced greater fluorescent signal intensity than unconjugated particles in both 2D cells and 3D spheriods, indicating its potential as a probe for image-guided colorectal cancer surgery.

Keyphrases

living cells
induced apoptosis
quantum dots
cell cycle arrest
fluorescent probe
atrial fibrillation
minimally invasive
photodynamic therapy
high resolution
drug delivery
signaling pathway
oxidative stress
single molecule
coronary artery bypass
high intensity
cell proliferation
fluorescence imaging
label free