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In silico-guided sequence modifications of K-ras epitopes improve immunological outcome against G12V and G13D mutant KRAS antigens.

Allan Wee Ren NgPei Jun TanWinfrey Pui Yee HooDek Shen LiewMichelle Yee Mun TeoPui Yan SiakSze Man NgEe Wern TanRaha Abdul RahimRenee Lay Hong LimAdelene Ai Lian SongLionel Lian Aun In
Published in: PeerJ (2018)
In silico-guided predictions of mutated K-ras T- and B-cell epitopes were successful in identifying two immunogens with high predictive scores, Th-bias cytokine induction and IgG-specific stimulation. Developments of such immunogens are potentially useful for future immunotherapeutic and diagnostic applications against KRAS(+) malignancies, monoclonal antibody production, and various other research and development initiatives.
Keyphrases
  • wild type
  • monoclonal antibody
  • molecular docking
  • current status
  • quality improvement
  • immune response
  • molecular dynamics simulations