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Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022.

Ingra Morales ClaroCamila Malta RomanoDarlan da Silva CandidoEvelyn Lepka de LimaJosé Angelo Lauletta LindosoMariana Severo RamundoFilipe Romero Rebello MoreiraLuiz Alberto Costa BarraLuciana Marques Sansão BorgesLucas Alberto MedeirosMarcia Y S TomishigeTomas MoutinhoAnderson José Dias da SilvaCamila Cristina Martini RodriguesLuiz Cesar Fernandes de AzevedoLucy Santos Villas-BoasCamila Alves Maia da SilvaThaís Moura ColettiErika Regina ManuliAine O'TooleJoshua QuickNicholas LomanAndrew RambautNuno R FariaClaudia Figueiredo-MelloEster Cerdeira Sabino
Published in: Revista do Instituto de Medicina Tropical de Sao Paulo (2022)
Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.
Keyphrases
  • single cell
  • antibiotic resistance genes
  • single molecule
  • disease virus
  • genome wide
  • cell free
  • dna methylation
  • gene expression
  • clinical practice
  • anaerobic digestion