Implementation of a MSRE ddPCR method for the detection of methylated WIF1 and NPY genes in colorectal cancer patients.

Livio TrentinDebora BasileElena LazzariElena FiettaAlice RossiFilomena GrazianiAlessandro CappettaFrancesca SimionatoEmanuele D'AmoreOmar PerbelliniGiuseppe Aprile

Published in: Tumori (2024)

We showed that this method is robust and sensitive also allowing the monitoring of CRC disease progression when applied to circulating free DNA samples from liquid biopsies, proving to be a fast and easy to implement assay to be used for the monitoring of the methylation pattern of clinically relevant target genes.

Keyphrases

genome wide
dna methylation
bioinformatics analysis
primary care
genome wide identification
healthcare
circulating tumor
high throughput
single molecule
ionic liquid
cell free
quality improvement
gene expression
genome wide analysis
label free
ultrasound guided
transcription factor