Hypoxia with Wharton's jelly mesenchymal stem cell coculture maintains stemness of umbilical cord blood-derived CD34+ cells.
Dewan ZhaoLingjia LiuQiang ChenFangfang WangQiuyang LiQiang ZengJingcao HuangMaowen LuoWenxian LiYuhuan ZhengTing LiuPublished in: Stem cell research & therapy (2018)
Our data demonstrate that cord blood HSPCs maintain stemness better under hypoxia than normoxia with WJ-MSC coculture, partially due to the increased secretion of VEGF, decreased secretion of IL-6 by WJ-MSCs, and selective activation of stem cell signal pathways in HSPCs. This suggests that the oxygenation may not only be a physiological regulatory factor but also a cell engineering tool in HSPC research, and this may have important translational and clinical implications.
Keyphrases
- umbilical cord
- stem cells
- mesenchymal stem cells
- cord blood
- cell therapy
- endothelial cells
- induced apoptosis
- epithelial mesenchymal transition
- bone marrow
- cell cycle arrest
- single cell
- vascular endothelial growth factor
- big data
- transcription factor
- oxidative stress
- signaling pathway
- blood flow
- cell death
- machine learning
- artificial intelligence
- cell proliferation
- data analysis