Hydrazide-based reactive matrices for the sensitive detection of aldehydes and ketones by MALDI mass spectrometry imaging.
Henrik LodénLuke S SchembriAnna NilssonIbrahim KayaReza ShariatgorjiLuke R OdellPer E AndrenPublished in: Chemical communications (Cambridge, England) (2024)
A one-step, on-tissue chemical derivatisation method for MALDI mass spectrometry imaging was found to improve the detectability of aldehydes and ketones by charge-tagging. The developed reactive matrices, containing a UV-chromophore, ionisable moiety and hydrazide group, showed an equal or higher detection efficiency than Girard's reagent P, enabling improved imaging of brain metabolites without the need for additional co-matrices.
Keyphrases
- mass spectrometry
- high resolution
- sensitive detection
- liquid chromatography
- gas chromatography
- high performance liquid chromatography
- loop mediated isothermal amplification
- capillary electrophoresis
- ms ms
- white matter
- fluorescence imaging
- multiple sclerosis
- tandem mass spectrometry
- solid phase extraction
- aqueous solution