Protective potential of royal jelly against cadmium-induced infertility in male rats.
Mohamed Mohamed AhmedSamir A El-ShazlyMohamed E AlkafafyAlaa A MohamedAhmed A MousaPublished in: Andrologia (2018)
This study aimed to investigate the protective potential of Royal jelly (RJ) against cadmium (Cd)-induced testicular dysfunction in rats. Thirty-five adult male Wistar rats were assigned into five groups. G I; (control) injected intraperitoneally with saline, G II injected intraperitoneally with a single dose of CdCl2 (1 mg/kg BW), G III received RJ (100 mg/kg BW/day) orally, G IV was pre-treated with RJ for 1 week then, treated with CdCl2 , and G V was co-treated with RJ and CdCl2 . After day 56, serum and tissue samples were collected and analysed. The results showed decreased serum testosterone, luteinising hormone (LH), follicle-stimulating hormone (FSH), superoxide dismutase, glutathione reductase, sperm motility and count while increased malondialdehyde, nitric oxide, tumour necrosis factor-α (TNF-α) and sperm abnormalities, along with a severely damaged seminiferous tubules epithelium with cytoplasmic and nuclear disruptions following Cd toxicity. Additionally, Cd stimulated testicular mRNA expression of TNF-α while inhibited those of steroidogenic acute regulatory protein, cytochrome P450 cholesterol side chain cleavage enzyme androgen binding protein, FSH-receptor, LH-receptor, androgen receptor, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, and cytochrome P450 17A1. These negative alterations of cadmium were greatly reduced by RJ treatment. This study concluded that RJ protects against Cd-induced testicular toxicity.
Keyphrases
- binding protein
- nitric oxide
- high glucose
- diabetic rats
- oxidative stress
- drug induced
- rheumatoid arthritis
- germ cell
- nk cells
- hydrogen peroxide
- endothelial cells
- adipose tissue
- type diabetes
- liver failure
- newly diagnosed
- hepatitis b virus
- human health
- bone marrow
- respiratory failure
- acute respiratory distress syndrome
- young adults
- umbilical cord
- study protocol