Complementary Powerful Techniques for Investigating the Interactions of Proteins with Porous TiO 2 and Its Hybrid Materials: A Tutorial Review.
Yihui DongWei-Feng LinAatto LaaksonenXiaoyan JiPublished in: Membranes (2022)
Understanding the adsorption and interaction between porous materials and protein is of great importance in biomedical and interface sciences. Among the studied porous materials, TiO 2 and its hybrid materials, featuring distinct, well-defined pore sizes, structural stability and excellent biocompatibility, are widely used. In this review, the use of four powerful, synergetic and complementary techniques to study protein-TiO 2- based porous materials interactions at different scales is summarized, including high-performance liquid chromatography (HPLC), atomic force microscopy (AFM), surface-enhanced Raman scattering (SERS), and Molecular Dynamics (MD) simulations. We expect that this review could be helpful in optimizing the commonly used techniques to characterize the interfacial behavior of protein on porous TiO 2 materials in different applications.
Keyphrases
- molecular dynamics
- high performance liquid chromatography
- atomic force microscopy
- metal organic framework
- tissue engineering
- high speed
- simultaneous determination
- mass spectrometry
- protein protein
- highly efficient
- tandem mass spectrometry
- density functional theory
- ms ms
- amino acid
- binding protein
- solid phase extraction
- gold nanoparticles
- molecular dynamics simulations