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Paper-based Transwell assays: an inexpensive alternative to study cellular invasion.

Rachael M KenneyAdam LoeserNathan A WhitmanMatthew R Lockett
Published in: The Analyst (2019)
Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.
Keyphrases
  • extracellular matrix
  • high throughput
  • cell migration
  • single cell
  • rna seq
  • cell therapy
  • mesenchymal stem cells