Cancer Antigen Discovery Is Enabled by RNA Sequencing of Highly Purified Malignant and Nonmalignant Cells.
Martin J ScurrAlex Greenshields-WatsonEmma CampbellMichelle S SomervilleYuan ChenSarah L Hulin-CurtisStephanie E A BurnellJames A DaviesMichael M DaviesRachel HargestSimon PhillipsAdam D ChristianKevin E AshelfordRobert AndrewsAlan L ParkerRichard James StantonAwen GallimoreAndrew GodkinPublished in: Clinical cancer research : an official journal of the American Association for Cancer Research (2020)
This study highlights how prior methods that sequence whole tumor fractions (i.e., inclusive of alive/dead stromal cells) for antigen identification may have limitations. Through tumor cell purification and sequencing, novel candidate TAAs have been identified for future immunotherapeutic targeting.