Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe.
Fangmei ZhangPinyi MaXinyu DengYing SunXinghua WangDaqian SongPublished in: Mikrochimica acta (2018)
Glutathione-capped water-soluble CuInS/ZnS quantum dots (QDs) were prepared by a microwave-assisted method. Their fluorescence, with excitation/emission peaks at 380/570 nm, is found to be quenched by hydrogen peroxide (H2O2) that is produced by the uricase catalyzed oxidation of uric acid (UA) and oxygen. The findings are used in a quenchometric method for the determination of UA. The effects of different ligands on the QDs, of pH value, buffers, enzyme ratio and reaction time were optimized. The detection limit for UA is 50 nM which is lower than other QD-based method, and the detection ranges extends from 0.25-4.0 μM. The assay is simple and sensitive, and no further modification of the QDs is required. Graphical abstract ᅟ.
Keyphrases
- uric acid
- quantum dots
- water soluble
- hydrogen peroxide
- energy transfer
- fluorescent probe
- metabolic syndrome
- sensitive detection
- nitric oxide
- living cells
- loop mediated isothermal amplification
- photodynamic therapy
- solid phase extraction
- real time pcr
- label free
- molecularly imprinted
- single molecule
- high throughput
- high resolution
- tandem mass spectrometry