Distribution of H3K27me3, H3K9me3, and H3K4me3 along autophagy-related genes highly expressed in starved zebrafish myotubes.
Peggy R BigaMary N LatimerJacob Michael FroehlichJean-Charles GabillardIban SeiliezPublished in: Biology open (2017)
The zebrafish (Danio rerio) remains the teleost fish of choice for biological investigations due to the vast array of molecular tools and resources available. To better understand the epigenetic regulation of autophagy, we utilized a primary myotube culture system generated from isolated myogenic precursor cells (MPCs) from zebrafish grown under starvation conditions using a media devoid of serum and amino acids. Here, we report starvation-induced regulation of several autophagy-related genes (atg) expression and profile the distribution of H3K27me3, H3K9me3, and H3K4me3 marks along lc3b, atg4b and p62/sqstm1 loci. These data support epigenetic regulation of autophagy in response to starvation that suggests a level of regulation that can be sustained for chronic conditions via chromatin modification.
Keyphrases
- cell death
- endoplasmic reticulum stress
- induced apoptosis
- signaling pathway
- oxidative stress
- cell cycle arrest
- genome wide
- amino acid
- gene expression
- skeletal muscle
- electronic health record
- transcription factor
- high throughput
- drug induced
- endothelial cells
- artificial intelligence
- cell proliferation
- solid phase extraction