A New Detergent for the Effective Decellularization of Bovine and Porcine Pericardia.
Martina TodescoSaima Jalil ImranTiago Moderno FortunatoDeborah SandrinGiulia BorileFilippo RomanatoMartina CasarinGermana GiuggioliFabio ConteMassimo MarchesanGino GerosaAndrea BagnoPublished in: Biomimetics (Basel, Switzerland) (2022)
Human and animal pericardia are among the most widely exploited materials suitable to repair damaged tissues in the cardiovascular surgery context. Autologous, xenogeneic (chemically treated) and homologous pericardia are largely utilized, but they do exhibit some crucial drawbacks. Any tissue treated with glutaraldehyde is known to be prone to calcification in vivo, lacks regeneration potential, has limited durability, and can result in cytotoxicity. Moreover, autologous tissues have limited availability. Decellularized biological tissues represent a promising alternative: decellularization removes cellular and nuclear components from native tissues and makes them suitable for repopulation by autologous cells upon implantation into the body. The present work aims to assess the effects of a new detergent, i.e., Tergitol, for decellularizing bovine and porcine pericardia. The decellularization procedure successfully removed cells, while preserving the histoarchitecture of the extracellular matrix. No cytotoxic effect was observed. Therefore, decellularized pericardia showed potential to be used as scaffold for cardiovascular tissue regeneration.
Keyphrases
- extracellular matrix
- induced apoptosis
- gene expression
- stem cells
- bone marrow
- cell cycle arrest
- cell therapy
- minimally invasive
- endothelial cells
- platelet rich plasma
- tissue engineering
- endoplasmic reticulum stress
- chronic kidney disease
- dna damage
- signaling pathway
- coronary artery bypass
- mesenchymal stem cells
- dna repair
- acute coronary syndrome
- risk assessment
- induced pluripotent stem cells
- atrial fibrillation
- newly diagnosed