An upgraded 2D nanosheet-based FRET biosensor: insights into avoiding background and eliminating effects of background fluctuations.

We demonstrated a new class of 2D nanosheet-based FRET biosensor utilizing vertically oriented MoS 2 nanosheets on a magnetic nanocarrier. Compared with the non-separated biosensor under identical conditions, this upgraded one can avoid the background signal of the system and eliminate the effects of background fluctuations, which produces more excellent detection methods .