Sterically stabilized siRNA:gold nanocomplexes enhance c-MYC silencing in a breast cancer cell model.
Aliscia N DanielsMoganavelli SinghPublished in: Nanomedicine (London, England) (2019)
Aim: To produce sterically stabilized and functionalized gold nanoparticles (AuNPs) for efficient delivery of siRNA for c-MYC silencing in vitro. Materials & methods: Synthesized AuNPs were functionalized with chitosan and PEG400 and PEG2000, morphologically and chemically characterized, and assessed for cytotoxicity and gene silencing in vitro. Results & discussion: AuNPs presented as spherical particles in the nanometer size range and successfully bound and protected the siRNA against degradation and were well tolerated in the breast adenocarcinoma (MCF-7) cell line. Nanoparticle-mediated gene knockdown studies revealed enhanced levels of c-MYC gene silencing with more than 90% reduction of MYC protein levels. Conclusion: These nanoformulations show enhanced potential for siRNA-mediated gene silencing in human breast cancer cells in vitro.
Keyphrases
- cancer therapy
- drug delivery
- breast cancer cells
- gold nanoparticles
- hyaluronic acid
- endothelial cells
- quantum dots
- squamous cell carcinoma
- transcription factor
- gene expression
- genome wide
- dna methylation
- amino acid
- wound healing
- genome wide identification
- tandem mass spectrometry
- simultaneous determination
- iron oxide