Wortmannin and Wortmannine Analogues from an Undescribed Niesslia sp.
Nicole M DischlerLijian XuYan LiConnie B NicholsJ Andrew AlspaughGerald F BillsJames B GloerPublished in: Journal of natural products (2019)
In the course of our studies of coprophilous fungi as sources of antifungal agents, a strain of an undescribed species in the genus Niesslia (TTI-0426) was isolated from horse dung collected in Texas. An extract from fermentation cultures of this strain afforded two new antifungal wortmannin derivatives, wortmannins C and D (1 and 2), as well as four additional new related compounds, wortmannines B1-B4 (3-6), containing an unusual ring system. The structures of these metabolites were established mainly by analysis of HRESIMS and 2D NMR data. Relative configurations were assigned using NOESY data, and the structure assignments were supported by NMR comparison with similar compounds. Wortmannins C and D showed activity against Cryptococcus neoformans and Candida albicans in disk assays, but low MIC potency observed for 1 was suggested to be due in part to efflux processes on the basis of assay results for a Schizosaccharomyces pombe efflux mutant in comparison to wild-type.
Keyphrases
- candida albicans
- wild type
- high resolution
- biofilm formation
- magnetic resonance
- electronic health record
- high throughput
- big data
- solid state
- oxidative stress
- drinking water
- molecular docking
- structure activity relationship
- mass spectrometry
- escherichia coli
- pseudomonas aeruginosa
- artificial intelligence
- case control
- machine learning
- clinical evaluation
- anti inflammatory
- data analysis