Simultaneous Determination of Xanthine and Hypoxanthine Using Polyglycine/rGO-Modified Glassy Carbon Electrode.
Ting WangLin ZhangChengyu ZhangDongmei DengDejia WangLiqiang LuoPublished in: Molecules (Basel, Switzerland) (2023)
A novel electrochemical sensor was developed for selective and sensitive determination of xanthine (XT) and hypoxanthine (HX) based on polyglycine (p-Gly) and reduced graphene oxide (rGO) modified glassy carbon electrode (GCE). A mixed dispersion of 7 μL of 5 mM glycine and 1 mg/mL GO was dropped on GCE for the fabrication of p-Gly/rGO/GCE, followed by cyclic voltammetric sweeping in 0.1 M phosphate buffer solution within -0.45~1.85 V at a scanning rate of 100 mV·s -1 . The morphological and electrochemical features of p-Gly/rGO/GCE were investigated by scanning electron microscopy and cyclic voltammetry. Under optimal conditions, the linear relationship was acquired for the simultaneous determination of XT and HX in 1-100 μM. The preparation of the electrode was simple and efficient. Additionally, the sensor combined the excellent conductivity of rGO and the polymerization of Gly, demonstrating satisfying simultaneous sensing performance to both XT and HX.
Keyphrases
- reduced graphene oxide
- simultaneous determination
- gold nanoparticles
- electron microscopy
- solid phase extraction
- molecularly imprinted
- liquid chromatography tandem mass spectrometry
- high performance liquid chromatography
- tandem mass spectrometry
- ultra high performance liquid chromatography
- liquid chromatography
- high resolution
- carbon nanotubes
- solid state
- uric acid
- ionic liquid
- gas chromatography
- ms ms
- high resolution mass spectrometry
- metabolic syndrome
- label free