Assessing the ratio of Bacillus spores and vegetative cells by shotgun proteomics.
Charlotte MappaOlivier PibleJean ArmengaudBéatrice Alpha-BazinPublished in: Environmental science and pollution research international (2018)
Mass spectrometry for rapid identification of microorganisms is expanding over the last years because this approach is quick. This methodology provides a decisive interest to fight against bioterrorism as it is applicable whatever the pathogen to be considered and often allows subtyping which may be crucial for confirming a massive and widespread attack with biological agents. Here, we present a methodology based on next-generation proteomics and tandem mass spectrometry for discovering numerous protein biomarkers allowing the discrimination of spores and vegetative cells of Bacillus atrophaeus, a biowarfare simulant. We propose a global quantitative evaluation of the two groups of discriminant biomarkers based on their aggregated normalized spectral abundance factors.
Keyphrases
- mass spectrometry
- liquid chromatography
- tandem mass spectrometry
- induced apoptosis
- gas chromatography
- high performance liquid chromatography
- cell cycle arrest
- high resolution
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- oxidative stress
- optical coherence tomography
- magnetic resonance imaging
- magnetic resonance
- signaling pathway
- cell death
- solid phase extraction
- cell proliferation
- label free
- candida albicans
- bacillus subtilis
- computed tomography
- ms ms
- small molecule
- amino acid
- wastewater treatment
- antibiotic resistance genes