Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica.
Thomas BeneytonStéphane ThomasAndrew D GriffithsJean-Marc NicaudAntoine DrevelleTristan RossignolPublished in: Microbial cell factories (2017)
Yarrowia lipolytica was used to express fungal genes encoding hydrolytic enzymes of interest. We developed a successful droplet-based microfluidic platform for the high-throughput screening (105 strains/h) of Y. lipolytica based on enzyme secretion and activity. This approach provides highly efficient tools for the HTS of recombinant enzymatic activities. This should be extremely useful for discovering new biocatalysts via directed evolution or protein engineering approaches and should lead to major advances in microbial cell factory development.
Keyphrases
- single cell
- highly efficient
- high throughput
- saccharomyces cerevisiae
- escherichia coli
- microbial community
- genome wide
- cell wall
- hydrogen peroxide
- circulating tumor cells
- protein protein
- cell therapy
- binding protein
- mesenchymal stem cells
- amino acid
- stem cells
- nitric oxide
- dna methylation
- cell free
- small molecule
- label free
- transcription factor