Joint application of metagenomic next-generation sequencing and histopathological examination for the diagnosis of pulmonary infectious disease.
Linhui YangKaige WangYalun LiWei-Min LiDan LiuPublished in: Microbiology spectrum (2023)
The diagnosis of some pulmonary infectious diseases and their pathogens is very difficult. A more precise diagnosis of pulmonary infectious diseases can help clinicians use proper antibiotics as well as reduce the development of drug-resistant bacteria. In this study, we performed both mNGS and pathology on lung puncture biopsy tissue from patients and found that combined mNGS and histopathology testing was significantly more effective than histopathology testing alone in detecting infectious diseases and identifying infectious diseases. In addition, the combined approach improves the detection rate of pathogenic microorganisms in infectious diseases and can be used to guide precision clinical treatment.
Keyphrases
- infectious diseases
- drug resistant
- pulmonary hypertension
- multidrug resistant
- end stage renal disease
- ejection fraction
- acinetobacter baumannii
- newly diagnosed
- ultrasound guided
- peritoneal dialysis
- gene expression
- dna methylation
- copy number
- antimicrobial resistance
- quantum dots
- loop mediated isothermal amplification
- wastewater treatment