An automated workflow to screen alkene reductases using high-throughput thin layer chromatography.
Brett M GarabedianCorey W MeadowsFlorence MingardonJoel M GuentherTristan de RondRaya AbourjeilyTaek Soon LeePublished in: Biotechnology for biofuels (2020)
With appropriate quantitation methods, this workflow can be used to screen polyprenyl reductase activity and can be readily adapted to analyze broader catalyst libraries whose products are amenable to TLC analysis.
Keyphrases
- high throughput
- mass spectrometry
- tandem mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- single cell
- electronic health record
- ms ms
- ionic liquid
- room temperature
- liquid chromatography tandem mass spectrometry
- highly efficient
- reduced graphene oxide
- high resolution
- carbon dioxide
- solid phase extraction
- visible light