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Nonclassical Kinetics of Clonal yet Heterogeneous Enzymes.

Seong Jun ParkSanggeun SongIn-Chun JeongHye Ran KohJi-Hyun KimJaeyoung Sung
Published in: The journal of physical chemistry letters (2017)
Enzyme-to-enzyme variation in the catalytic rate is ubiquitous among single enzymes created from the same genetic information, which persists over the lifetimes of living cells. Despite advances in single-enzyme technologies, the lack of an enzyme reaction model accounting for the heterogeneous activity of single enzymes has hindered a quantitative understanding of the nonclassical stochastic outcome of single enzyme systems. Here we present a new statistical kinetics and exactly solvable models for clonal yet heterogeneous enzymes with possibly nonergodic state dynamics and state-dependent reactivity, which enable a quantitative understanding of modern single-enzyme experimental results for the mean and fluctuation in the number of product molecules created by single enzymes. We also propose a new experimental measure of the heterogeneity and nonergodicity for a system of enzymes.
Keyphrases
  • living cells
  • gene expression
  • high resolution
  • healthcare
  • dna methylation
  • mass spectrometry
  • single cell
  • social media
  • genome wide