The Osteogenic Differentiation Effect of the FN Type 10-Peptide Amphiphile on PCL Fiber.
Ye-Rang YunHae-Won KimJun-Hyeog JangPublished in: International journal of molecular sciences (2018)
The fibronectin type 10-peptide amphiphile (FNIII10-PA) was previously genetically engineered and showed osteogenic differentiation activity on rat bone marrow stem cells (rBMSCs). In this study, we investigated whether FNIII10-PA demonstrated cellular activity on polycaprolactone (PCL) fibers. FNIII10-PA significantly increased protein production and cell adhesion activity on PCL fibers in a dose-dependent manner. In cell proliferation results, there was no effect on cell proliferation activity by FNIII10-PA; however, FNIII10-PA induced the osteogenic differentiation of MC3T3-E1 cells via upregulation of bone sialoprotein (BSP), collagen type I (Col I), osteocalcin (OC), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) mitochondrial RNA (mRNA) levels; it did not increase the alkaline phosphatase (ALP) mRNA level. These results indicate that FNIII10-PA has potential as a new biomaterial for bone tissue engineering applications.
Keyphrases
- tissue engineering
- cell proliferation
- bone marrow
- transcription factor
- stem cells
- mesenchymal stem cells
- bone mineral density
- oxidative stress
- cell adhesion
- cell cycle
- induced apoptosis
- binding protein
- risk assessment
- cell therapy
- body composition
- dna binding
- cell death
- postmenopausal women
- climate change
- human health
- wound healing
- stress induced