Rapid detection and quantification of plasmid-mediated colistin resistance genes (mcr-1 to mcr-5) by real-time PCR in bacterial and environmental samples.
R TolosiI ApostolakosA LaconiL CarraroG GrilliP CagnardiAlessandra PiccirilloPublished in: Journal of applied microbiology (2020)
Colistin is an antimicrobial commonly used in animals and has recently emerged as a last-resort treatment in humans. Plasmid-mediated mcr genes confer resistance to colistin and represent a major threat for public health since they can be easily disseminated through horizontal gene transfer. The rapid and sensitive detection of mcr genes is of utmost necessity.
Keyphrases
- escherichia coli
- loop mediated isothermal amplification
- sensitive detection
- klebsiella pneumoniae
- real time pcr
- genome wide
- genome wide identification
- public health
- multidrug resistant
- bioinformatics analysis
- genome wide analysis
- quantum dots
- dna methylation
- staphylococcus aureus
- transcription factor
- copy number
- drug resistant
- gene expression
- crispr cas
- label free
- climate change
- smoking cessation