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A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing.

François F BarbierTinashe G ChabikwaMuhammad Umair AhsanStacey E CookRosanna PowellMilos TanurdzicChristine Anne Beveridge
Published in: Plant methods (2019)
This method enables safer and faster DNA and RNA extraction from recalcitrant species, thus facilitating future work on tropical trees.
Keyphrases
  • gene expression
  • climate change
  • dna methylation
  • oxidative stress
  • single cell
  • circulating tumor
  • nucleic acid
  • current status
  • single molecule
  • genetic diversity