Proteomic characterization of hydatid cyst fluid: two-dimensional electrophoresis (2-DE) setup through optimizing protein extraction.

Bahareh Sedaghat Homa Hajjaran Fatemeh Sadat Sadjjadi Soudabeh Heidari Seyed Mahmoud Sadjjadi

Published in: BMC research notes (2021)

The 2-DE of TCA/Acetone products showed a lot of smears in the background of gels; TCA/Acetone with dialysis showed greatly reduced smears while the 2-D Clean-up Kit together with dialysis showed sharp spots and least smears. Three-dimensional images of separated spots created by Progenesis SameSpots software showed the best result was achieved by 2-D Clean-up Kit and dialysis.

Keyphrases

chronic kidney disease
end stage renal disease
peritoneal dialysis
optical coherence tomography
amino acid
machine learning