Jchain - DTR Mice Allow for Diphtheria Toxin-Mediated Depletion of Antibody-Secreting Cells and Evaluation of Their Differentiation Kinetics.
KimAnh T PioliMatthew RitchieHira HaqPeter D PioliPublished in: bioRxiv : the preprint server for biology (2024)
Antibody-secreting cells (ASCs) are generated following B cell activation and constitutively secrete antibodies. As such, ASCs are key mediators of humoral immunity whether it be in the context of pathogen exposure, vaccination or even homeostatic clearance of cellular debris. Therefore, understanding basic tenants of ASC biology such as their differentiation kinetics following B cell stimulation is of importance. Towards that aim, we developed a mouse model which expresses simian HBEGF (a.k.a., diphtheria toxin receptor ( DTR )) under the control of the endogenous Jchain locus (or J-DTR). ASCs from these mice expressed high levels of cell surface DTR and were acutely depleted following diphtheria toxin treatment. Furthermore, proof-of-principle experiments demonstrated the ability to use these mice to track ASC reconstitution following depletion in 3 distinct organs. Overall, J-DTR mice provide a new and highly effective genetic tool allowing for the study of ASC biology in a wide range of potential applications.
Keyphrases
- escherichia coli
- high fat diet induced
- induced apoptosis
- mouse model
- cell surface
- cell cycle arrest
- type diabetes
- risk assessment
- wild type
- nlrp inflammasome
- signaling pathway
- insulin resistance
- adipose tissue
- endoplasmic reticulum stress
- genome wide
- cell proliferation
- climate change
- skeletal muscle
- human health
- smoking cessation