Recent Progress of Small-Molecule Ratiometric Fluorescent Probes for Peroxynitrite in Biological Systems.
Qingqing MaShanlin XuZhaodong ZhaiKai WangXueli LiuHaibin XiaoShuping ZhuoYuying LiuPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2022)
Peroxynitrite (ONOO - ) as a major reactive oxygen species plays important roles in cellular signal transduction and homeostatic regulation. Precise detection of ONOO - in biological systems is vital for exploring its physiological and pathological function. Among numerous detection methods, fluorescence imaging technology using fluorescent probes offers some advantages, including simple operation, high sensitivity and selectivity, as well as real-time and nondestructive detection. In particular, ratiometric fluorescent probes, in which the built-in calibration of the two emission bands prevents interference from the biological environment, have been extensively employed to monitor the fluctuation of bioactive species. In this review, we will discuss small-molecule ratiometric fluorescent probes for ONOO - in live cells or in vivo, which involves chemical structures, response mechanisms, and biological applications. Moreover, the challenges and future prospects of ONOO - -responsive ratiometric fluorescent probe are also proposed.
Keyphrases
- living cells
- fluorescent probe
- small molecule
- fluorescence imaging
- single molecule
- loop mediated isothermal amplification
- reactive oxygen species
- label free
- real time pcr
- protein protein
- induced apoptosis
- current status
- photodynamic therapy
- cell cycle arrest
- signaling pathway
- drug delivery
- cell proliferation
- oxidative stress
- mass spectrometry